Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"

Registro completo

Título

Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"

Autor(es)

Gutierrez, María de la Paz; Damron, F. Heath; Sisti, Federico; Fernández, Julieta

Afiliación(es) del/de los autor(es)

Gutierrez, María de la Paz. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Sisti, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fernández, Julieta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina

Resumen

Bordetella bronchiseptica is a pathogenic bacterium that causes respiratory infections in mammals. Adhesins, toxins and secretion systems necessary for infection are regulated by the two-component system BvgAS. When the BvgAS system is inactive, there is no transcription of virulence-activated genes and virulence-repressed genes (vrg) are expressed. The regulation of some vrgs in B. bronchiseptica is dependent upon the virulence-activated gene bvgR. Although having a regulatory role, no DNA-binding domain is described for BvgR. Instead, it contains an EAL domain, usually found in cyclic-di-GMP-specific phosphodiesterases (PDE). Cyclic-di-GMP (c-di-GMP) is a bacterial second messenger that regulates multiple phenotypes in bacteria, including B. bronchiseptica. The current study aimed to deepen our knowledge about BvgR. We employed RNA-seq analysis to define the BvgR regulon and then we investigated the phenotypes in which BvgR regulation might be involved such as biofilm formation, cytotoxicity, and virulence. Our result revealed that BvgR inhibits biofilm formation and flagellin expression in virulent phase. Although BvgR has long been considered a repressor protein, our results show that it also upregulates almost 100 genes. This regulation is likely indirect, as BvgR lacks a DNA-binding domain. Notably, among the upregulated genes, we identified 15 associated with the type three secretion system. Consistent with these findings, a B. bronchiseptica strain deficient in bvgR was less cytotoxic than the wild-type strain, elicited a milder immune response, and was less able to persist in the lower respiratory tract of mice.
Facultad de Ciencias Exactas

Año de publicación

2024

Idioma

español

Formato (Tipo MIME)

application/zip
El material es resultado del trabajo citado. Se emplearon técnicas de identificación y cuantificación masiva de proteína (Shot gun proteomics, Orbitrap). Además, se utilizaron técnicas de microbiología clásica: determinación de formación de biofilm, movilidad, etc.

Clasificación temática de acuerdo a la FORD

Ciencias biológicas

Materia

Ciencias Exactas; Biofilm; Bordetella;

Condiciones de uso

Disponible en acceso abierto bajo licencia Creative Commons http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)

Repositorio digital

SEDICI (UNLP) - Universidad Nacional de La Plata