{"id":"SEDICI_ce66e72ad491e9c405c910c5cd2ec22a","dc:title":"Supplementary material from \"BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica\"","dc:creator":"Fern\u00e1ndez, Julieta","dc:date":"2024","dc:description":["Bordetella bronchiseptica is a pathogenic bacterium that causes respiratory infections in mammals. Adhesins, toxins and secretion systems necessary for infection are regulated by the two-component system BvgAS. When the BvgAS system is inactive, there is no transcription of virulence-activated genes and virulence-repressed genes (vrg) are expressed. The regulation of some vrgs in B. bronchiseptica is dependent upon the virulence-activated gene bvgR. Although having a regulatory role, no DNA-binding domain is described for BvgR. Instead, it contains an EAL domain, usually found in cyclic-di-GMP-specific phosphodiesterases (PDE). Cyclic-di-GMP (c-di-GMP) is a bacterial second messenger that regulates multiple phenotypes in bacteria, including B. bronchiseptica. The current study aimed to deepen our knowledge about BvgR. We employed RNA-seq analysis to define the BvgR regulon and then we investigated the phenotypes in which BvgR regulation might be involved such as biofilm formation, cytotoxicity, and virulence. Our result revealed that BvgR inhibits biofilm formation and flagellin expression in virulent phase. Although BvgR has long been considered a repressor protein, our results show that it also upregulates almost 100 genes. This regulation is likely indirect, as BvgR lacks a DNA-binding domain. Notably, among the upregulated genes, we identified 15 associated with the type three secretion system. Consistent with these findings, a B. bronchiseptica strain deficient in bvgR was less cytotoxic than the wild-type strain, elicited a milder immune response, and was less able to persist in the lower respiratory tract of mice.","Facultad de Ciencias Exactas"],"dc:format":["application\/zip","El material es resultado del trabajo citado. Se emplearon t\u00e9cnicas de identificaci\u00f3n y cuantificaci\u00f3n masiva de prote\u00edna (Shot gun proteomics, Orbitrap). Adem\u00e1s, se utilizaron t\u00e9cnicas de microbiolog\u00eda cl\u00e1sica: determinaci\u00f3n de formaci\u00f3n de biofilm, movilidad, etc."],"dc:language":["spa"],"dc:type":"dataset","dc:subject":["Ciencias Exactas","Biofilm","Bordetella"],"dc:rights":["info:eu-repo\/semantics\/openAccess","http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/","Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)"],"dc:identifier":"https:\/\/repositoriosdigitales.mincyt.gob.ar\/vufind\/Record\/SEDICI_ce66e72ad491e9c405c910c5cd2ec22a"}