Dataset for Cyclic di-GMP regulates type three secretion system and virulence in Bordetella bronchiseptica

Registro completo

Título

Dataset for Cyclic di-GMP regulates type three secretion system and virulence in Bordetella bronchiseptica

Autor(es)

Gutierrez, María de la Paz; Wong, Ting; Damron, Frederick; Fernández, Julieta; Sisti, Federico

Afiliación(es) del/de los autor(es)

Fernández, Julieta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina.
Gutierrez, María de la Paz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular, Argentina.
Gutierrez, María de la Paz. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Sisti, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina

Resumen

The second messenger c-di-GMP is a ubiquitous molecule in bacteria that regulates diverse phenotypes. Among them, motility and biofilm formation are the most studied. Furthermore, c-di-GMP has been suggested to regulate virulence factors, therefore making it important for pathogenesis. Previously we described that c-di-GMP regulates biofilm formation and swimming motility in Bordetella bronchiseptica. Here we present a multi-omic approach on B. bronchiseptica strains expressing different cytoplasmic c-di-GMP levels, including RNA-seq and shotgun proteomics with label-free quantification. We detected 64 proteins significantly up- or down-regulated in either low or high c-di-GMP levels and 358 genes differentially expressed between high c-di-GMP levels and the wild type strain. Among them we found stress-related proteins, nitrogen metabolism enzymes, phage-related genes and virulence factors. Interestingly, we observed that a virulence factor like the type three secretion system (TTSS) was regulated by c-di-GMP. B. bronchiseptica with high c-di-GMP levels showed significantly lower levels of TTSS components like Bsp22, BopN and Bcr4. These findings were confirmed by independent methods as q-RT PCR and western blot. Higher intracellular levels of c-di-GMP correlated with impaired capacity to induce cytotoxicity in a eukaryotic cell in vitro and with attenuated virulence in a murine model. This work presents data that will support the role that the second messenger c-di-GMP plays in the pathogenesis of Bordetellae.
Facultad de Ciencias Exactas
Consejo Nacional de Investigaciones Científicas y Técnicas

Año de publicación

2021

Idioma

inglés

Formato (Tipo MIME)

application/vnd.ms-excel

Clasificación temática de acuerdo a la FORD

Ciencias biológicas

Materia

Ciencias Exactas;

Condiciones de uso

Disponible en acceso abierto bajo licencia Creative Commons http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)

Repositorio digital

SEDICI (UNLP) - Universidad Nacional de La Plata