{"id":"SEDICI_350d2a802d74e10ca6e8a009359b8f0a","dc:title":"Dataset for Cyclic di-GMP regulates type three secretion system and virulence in <i>Bordetella bronchiseptica<\/i>","dc:creator":"Sisti, Federico","dc:date":"2024","dc:description":["The second messenger c-di-GMP is a ubiquitous molecule in bacteria that regulates diverse phenotypes. Among them, motility and biofilm formation are the most studied. Furthermore, c-di-GMP has been suggested to regulate virulence factors, therefore making it important for pathogenesis. Previously we described that c-di-GMP regulates biofilm formation and swimming motility in Bordetella bronchiseptica. Here we present a multi-omic approach on B. bronchiseptica strains expressing different cytoplasmic c-di-GMP levels, including RNA-seq and shotgun proteomics with label-free quantification. We detected 64 proteins significantly up- or down-regulated in either low or high c-di-GMP levels and 358 genes differentially expressed between high c-di-GMP levels and the wild type strain. Among them we found stress-related proteins, nitrogen metabolism enzymes, phage-related genes and virulence factors. Interestingly, we observed that a virulence factor like the type three secretion system (TTSS) was regulated by c-di-GMP. B. bronchiseptica with high c-di-GMP levels showed significantly lower levels of TTSS components like Bsp22, BopN and Bcr4. These findings were confirmed by independent methods as q-RT PCR and western blot. Higher intracellular levels of c-di-GMP correlated with impaired capacity to induce cytotoxicity in a eukaryotic cell in vitro and with attenuated virulence in a murine model. This work presents data that will support the role that the second messenger c-di-GMP plays in the pathogenesis of Bordetellae.","Facultad de Ciencias Exactas","Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas"],"dc:format":["application\/vnd.ms-excel"],"dc:language":["eng"],"dc:type":"dataset","dc:subject":["Ciencias Exactas"],"dc:rights":["info:eu-repo\/semantics\/openAccess","http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/","Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)"],"dc:identifier":"https:\/\/repositoriosdigitales.mincyt.gob.ar\/vufind\/Record\/SEDICI_350d2a802d74e10ca6e8a009359b8f0a"}