Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets

Registro completo

Título

Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets

Autor(es)

Lencina, Florencia Aylen; Zbrun, María Virginia

Afiliación(es) del/de los autor(es)

Lencina, Florencia Aylen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Zbrun, María Virginia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina

Resumen

Background: This study aimed to assess the presence of thermotolerant Campylobacter resistant to ciprofloxacin and erythromycin in poultry slaughterhouses and retail markets, as well as to characterize their multidrug resistance profiles, genetic determinants, and clonal relationships. Methods: Samples were collected at slaughterhouses from caecal content (n= 270), neck skin (n= 270), and wastewater (n= 9) and at retail markets from breast skin (n=241). Isolates were obtained from mCCDA agar supplemented with ciprofloxacin (2 μg/ml) and identified as C. jejuni or C. coli by PCR. Agar microdilution test was used to determine the minimum inhibitory concentration for both ciprofloxacin and erythromycin and other critical antibiotics. Point mutations in gyrA (Thr86Ile) and 23S rRNA (A2075G), virulence genes (flaA, flhA, cadF, and cdt), and clonal relationships were assessed by PCR and PFGE. Results: At the slaughterhouses, thermotolerant Campylobacter resistant to erythromycin and ciprofloxacin were detected in 48.55% (107/549) of the samples, whereas 4.56% (11/241) of retail samples were positive. The Thr86Ile substitution in gyrA and the A2075G mutation in the 23S rRNA gene were detected in 92.97% and 89.84% of the isolates, respectively. Most isolates (>80%) were multidrug-resistant, harboured key virulence genes (flaA, flhA, and cadF). C. jejuni exhibited the highest prevalence of cdt genes (76.19%). There was substantial genotypic diversity among isolates, with broad distribution across the sampled matrices and sites. Conclusions: These findings highlight the circulation of multidrug-resistant and potentially virulent thermotolerant Campylobacter in the later stages of the poultry meat supply chain.

Año de publicación

2026

Idioma

español

Formato (Tipo MIME)

application/vnd.openxmlformats-officedocument.spreadsheetml.sheet

Clasificación temática de acuerdo a la FORD

Ciencia veterinaria

Condiciones de uso

Disponible en acceso abierto bajo licencia Creative Commons https://creativecommons.org/licenses/by-nc-sa/2.5/ar/

Repositorio digital

CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas