{"id":"CONICETDig_98dad8a232225df3001386c0bfb8e14a","dc:title":"Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets","dc:creator":"Zbrun, Mar\u00eda Virginia","dc:date":"2026","dc:description":["Background: This study aimed to assess the presence of thermotolerant Campylobacter resistant to ciprofloxacin and erythromycin in poultry slaughterhouses and retail markets, as well as to characterize their multidrug resistance profiles, genetic determinants, and clonal relationships. Methods: Samples were collected at slaughterhouses from caecal content (n= 270), neck skin (n= 270), and wastewater (n= 9) and at retail markets from breast skin (n=241). Isolates were obtained from mCCDA agar supplemented with ciprofloxacin (2 \u03bcg\/ml) and identified as C. jejuni or C. coli by PCR. Agar microdilution test was used to determine the minimum inhibitory concentration for both ciprofloxacin and erythromycin and other critical antibiotics. Point mutations in gyrA (Thr86Ile) and 23S rRNA (A2075G), virulence genes (flaA, flhA, cadF, and cdt), and clonal relationships were assessed by PCR and PFGE. Results: At the slaughterhouses, thermotolerant Campylobacter resistant to erythromycin and ciprofloxacin were detected in 48.55% (107\/549) of the samples, whereas 4.56% (11\/241) of retail samples were positive. The Thr86Ile substitution in gyrA and the A2075G mutation in the 23S rRNA gene were detected in 92.97% and 89.84% of the isolates, respectively. Most isolates (>80%) were multidrug-resistant, harboured key virulence genes (flaA, flhA, and cadF). C. jejuni exhibited the highest prevalence of cdt genes (76.19%). There was substantial genotypic diversity among isolates, with broad distribution across the sampled matrices and sites. Conclusions: These findings highlight the circulation of multidrug-resistant and potentially virulent thermotolerant Campylobacter in the later stages of the poultry meat supply chain."],"dc:format":["application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet"],"dc:language":["spa"],"dc:type":"dataset","dc:rights":["info:eu-repo\/semantics\/openAccess","https:\/\/creativecommons.org\/licenses\/by-nc-sa\/2.5\/ar\/"],"dc:identifier":"https:\/\/repositoriosdigitales.mincyt.gob.ar\/vufind\/Record\/CONICETDig_98dad8a232225df3001386c0bfb8e14a"}