Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa subunidad 4 (ND4) de Culex bidens y Culex interfor de Argentina

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Título

Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) y de 336 pares de bases del gen nicotinamida adenina dinucleótido deshidrogenasa subunidad 4 (ND4) de Culex bidens y Culex interfor de Argentina

Autor(es)

Laurito, Magdalena; Ayala, Ana Maria; Almiron, Walter Ricardo; Gardenal, Cristina Noemí

Afiliación(es) del/de los autor(es)

Laurito, Magdalena. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Laurito, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.
Ayala, Ana Maria. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina.
Ayala, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Diversidad y Ecología Animal; Argentina.
Almiron, Walter Ricardo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales.; Argentina.
Almiron, Walter Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.
Gardenal, Cristina Noemi. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina.
Gardenal, Cristina Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Diversidad y Ecología Animal; Argentina.

Resumen

Culex bidens and C. interfor, implicated in arbovirus transmission in Argentina, are sister species, only distinguishable by feature of the male genitalia; however, intermediate specimens of the species in sympatry have been found. Fourth-instar larvae and females of both species share apomorphic features, and this lack of clear distinction creates problems for specific identification. Geometric morphometric traits of these life stages also do not distinguish the species. The aim of the present study was to assess the taxonomic status of C. bidens and C. interfor using two mitochondrial genes and to determine the degree of their reproductive isolation using microsatellite loci. Sequences of the ND4 and COI genes were concatenated in a matrix of 993 nucleotides and used for phylogenetic and distance analyses. Bayesian and maximum parsimony inferences showed a well resolved and supported topology, enclosing sequences of individuals of C. bidens (0.83 BPP, 73 BSV) and C. interfor (0.98 BPP, 97 BSV) in a strong sister relationship. The mean K2P distance within C. bidens and C. interfor was 0.3% and 0.2%, respectively, and the interspecific variation was 2.3%. Bayesian clustering also showed two distinct mitochondrial lineages. All sequenced mosquitoes were successfully identified in accordance with the best close match algorithm. The low genetic distance values obtained indicate that the species diverged quite recently. Most morphologically intermediate specimens of C. bidens from Córdoba were heterozygous for the microsatellite locus GT51; the significant heterozygote excess observed suggests incomplete reproductive isolation. However, C. bidens and C. interfor should be considered good species: the ventral arm of the phallosome of the male genitalia and the ND4 and COI sequences are diagnostic characters.

Año de publicación

2022

Idioma

español

Formato (Tipo MIME)

application/zip

Clasificación temática de acuerdo a la FORD

Ciencias biológicas

Materia

Microsatélites; Introgresión; Especies crípticas; Taxonomía molecular;

Condiciones de uso

Disponible en acceso abierto

Repositorio digital

Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba