{"id":"RDUUNC_4c1bb61b4fdde0f4275f71899ef653bf","dc:title":"Travelling to the south: Phylogeographic spatial diffusion model in Monttea aphylla (Plantaginaceae), an endemic plant of the Monte Desert","dc:creator":"S\u00e9rsic, Alicia N.","dc:date":"2021","dc:description":["Effects of Pleistocene climatic oscillations on plant phylogeographic patterns are relatively well studied in forest, savanna and grassland biomes, but such impacts remain less explored on desert regions of the world, especially in South America. Here, we performed a phylogeographical study of Monttea aphylla, an endemic species of the Monte Desert, to understand the evolutionary history of vegetation communities inhabiting the South American Arid Diagonal. We obtained sequences of three chloroplast (trnS\u2013trnfM, trnH\u2013psbA and trnQ\u2013rps16) and one nuclear (ITS) intergenic spacers from 272 individuals of 34 localities throughout the range of the species. Population genetic and Bayesian coalescent analyses were performed to infer genealogical relationships among haplotypes, population genetic structure, and demographic history of the study species. Timing of demographic events was inferred using Bayesian Skyline Plot and the spatio-temporal patterns of lineage diversification was reconstructed using Bayesian relaxed diffusion models. Palaeo-distribution models (PDM) were performed through three different timescales to validate phylogeographical patterns. Twenty-five and 22 haplotypes were identified in the cpDNA and nDNA data, respectively. that clustered into two main genealogical lineages following a latitudinal pattern, the northern and the southern Monte (south of 35\u00b0 S). The northern Monte showed two lineages of high genetic structure, and more relative stable demography than the southern Monte that retrieved three groups with little phylogenetic structure and a strong signal of demographic expansion that would have started during the Last Interglacial period (ca. 120 Ka). The PDM and diffusion models analyses agreed in the southeast direction of the range expansion. Differential effect of climatic oscillations across the Monte phytogeographic province was observed in Monttea aphylla lineages. In northern Monte, greater genetic structure and more relative stable demography resulted from a more stable climate than in the southern Monte. Pleistocene glaciations drastically decreased the species area in the southern Monte, which expanded in a southeastern direction to the new available areas during the interglacial periods.","Los efectos de las oscilaciones clim\u00e1ticas del Pleistoceno sobre los patrones filogeogr\u00e1ficos de las plantas est\u00e1n relativamente bien estudiados en los biomas de bosque, sabana y pradera, pero tales impactos siguen siendo menos explorados en las regiones des\u00e9rticas del mundo, especialmente en Sudam\u00e9rica. Aqu\u00ed, realizamos un estudio filogeogr\u00e1fico de Monttea aphylla, una especie end\u00e9mica del desierto de Monte, para entender la historia evolutiva de las comunidades de vegetaci\u00f3n que habitan la Diagonal \u00c1rida sudamericana. Obtuvimos secuencias de tres cloroplastos (trnS-trnfM, trnH-psbA y trnQ-rps16) y un espaciador interg\u00e9nico nuclear (ITS) de 272 individuos de 34 localidades a lo largo del rango de la especie. Se realizaron an\u00e1lisis gen\u00e9ticos poblacionales y de coalescencia bayesiana para inferir las relaciones geneal\u00f3gicas entre los haplotipos, la estructura gen\u00e9tica de la poblaci\u00f3n y la historia demogr\u00e1fica de la especie estudiada. La cronolog\u00eda de los eventos demogr\u00e1ficos se dedujo mediante el Skyline Plot bayesiano y los patrones espacio-temporales de diversificaci\u00f3n de los linajes se reconstruyeron mediante modelos bayesianos de difusi\u00f3n relajada. Se realizaron modelos de paleodistribuci\u00f3n (PDM) a trav\u00e9s de tres escalas temporales diferentes para validar los patrones filogeogr\u00e1ficos. Se identificaron 25 y 22 haplotipos en los datos de ADNc y ADNn, respectivamente, que se agruparon en dos linajes geneal\u00f3gicos principales siguiendo un patr\u00f3n latitudinal, el Monte septentrional y el Monte meridional (al sur de 35\u00b0 S). El Monte septentrional mostr\u00f3 dos linajes de alta estructura gen\u00e9tica, y una demograf\u00eda m\u00e1s relativamente estable que el Monte meridional que recuper\u00f3 tres grupos con escasa estructura filogen\u00e9tica y una fuerte se\u00f1al de expansi\u00f3n demogr\u00e1fica que habr\u00eda comenzado durante el \u00faltimo per\u00edodo interglacial (ca. 120 Ka). Los an\u00e1lisis de los modelos PDM y de difusi\u00f3n coincidieron en la direcci\u00f3n sureste de la expansi\u00f3n del \u00e1rea de distribuci\u00f3n. Se observ\u00f3 un efecto diferencial de las oscilaciones clim\u00e1ticas a lo largo de la provincia fitogeogr\u00e1fica del Monte en los linajes de Monttea aphylla. En el norte del Monte, una mayor estructura gen\u00e9tica y una demograf\u00eda relativamente m\u00e1s estable fueron el resultado de un clima m\u00e1s estable que en el sur del Monte. Las glaciaciones del Pleistoceno redujeron dr\u00e1sticamente el \u00e1rea de la especie en el Monte meridional, que se expandi\u00f3 en direcci\u00f3n sureste hacia las nuevas \u00e1reas disponibles durante los periodos interglaciares."],"dc:format":["application\/zip"],"dc:language":["eng"],"dc:type":"dataset","dc:subject":["South American Arid Diagonal","Bayesian Skyline Plot","Monte phytogeographic province","Bayesian coalescent analyses","Last Interglacial period","Monte Desert Effects","Plant phylogeographic patterns","Diffusion models analyses","Monttea aphylla"],"dc:coverage":["name=South American"],"dc:rights":["info:eu-repo\/semantics\/openAccess"],"dc:publisher":"Plos One","dc:relation":["info:eu-repo\/semantics\/dataset\/doi\/10.1371\/journal.pone.0178827","info:eu-repo\/semantics\/reference\/hdl\/11086\/18112","info:eurepo\/semantics\/reference\/url\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0178827"],"dc:identifier":"https:\/\/repositoriosdigitales.mincyt.gob.ar\/vufind\/Record\/RDUUNC_4c1bb61b4fdde0f4275f71899ef653bf"}