Chaotic fluctuations in mitochondrial function under oxidative stress. SOD2 concentrations of 0.0216733 mM

Registro completo

Título

Chaotic fluctuations in mitochondrial function under oxidative stress. SOD2 concentrations of 0.0216733 mM

Autor(es)

Kembro, Jackelyn Melissa; Cortassa, Sonia; Lloyd, David; Sollot, Steven; Aon, Miguel

Afiliación(es) del/de los autor(es)

Kembro, Jackelyn Melissa. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Kembro, Jackelyn Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.
Cortassa, Sonia. National Institutes of Health. NIH · NIA Intramural Research Program; Estados Unidos.
Lloyd, David. Cardiff University. School of Biosciences 1; Inglaterra.
Sollot, Steven. Johns Hopkins University. Laboratory of Cardiovascular Science; Estados Unidos.
Aon, Miguel. Johns Hopkins University. Laboratory of Cardiovascular Science; Estados Unidos.

Resumen

Numerical integration of the ME-R model equations was performed with MatCont 2.4 in MATLAB 7.1, until steady-state solutions were obtained (i.e., when the magnitude of each time derivative was -10). Time series with duration of at least 6e6 ms were constructed by numerical integration of model equations. To allow transient states to vanish, the system was computed during a time lapse of 2 e8 ms. The solutions were then evaluated with the function deval.m in MATLAB R2017a to obtain a time series with constant sampling frequency at 1ms. All studies were performed using the parameter setting optimized in our previous work (Kembro et al. 2013. Biophys J 104(2):332-343; Kembro et al., 2014. Front Physiol 5:257), with ADPm = 0.01mM, i.e. consistent with energized mitochondria under state 4 respiration. The two .txt file represents the time reference and the time series of variables output of the model, in order from left to right column:1) Mitochondrial Ca+;2) Mitochondrial ADP; 3) Membrane potential; 4)Mitocondrial NADH; 5) Mitochondrial H+; 6) Mitochondrial Phosfate, Pi; 7) Isocitrate; 8) a-ketoglutarate; 9) Succinyl CoA; 10) Succinate; 11) Fumarate; 12) Malate; 13) Oxaloacetate; 14) NADPH; 15) Mitochondrial superoxide; 16) Extramitochondrial superoxide; 17) Mitochondrial hydrogen peroxide; 18) Extramitochondrial hydrogen peroxide; 19) Mitochondrial GSH; 20) Extramitochondrial GSH; 21)Mitochondrial GSSG; 22) Mitochondrial TrxSH2; 23) ExtramitochondrialTrxSH; 24)Mitochondrial PSSGm; 25) Extramitochondrial PSSG

Año de publicación

2018

Idioma

inglés

Formato (Tipo MIME)

application/zip

Clasificación temática de acuerdo a la FORD

Ciencia veterinaria

Materia

Animal Physiology - Systems; Molecular Biology; Superoxide dismutase activity; Respiration; Oxidative stress effect; Oxidative stress enzymes;

Condiciones de uso

Disponible en acceso abierto

Repositorio digital

Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba

Identificador alternativo

https://doi.org/10.6084/m9.figshare.1514982.v1

Publicación referenciada

http://hdl.handle.net/hdl/11086/17030