DACyTAr - Datos Primarios en Acceso Abierto de la Ciencia y la Tecnología Argentina
Chaotic fluctuations in mitochondrial function under oxidative stress. SOD2 concentrations of 0.0216733 mM
Registro completo
- Título
- Chaotic fluctuations in mitochondrial function under oxidative stress. SOD2 concentrations of 0.0216733 mM
- Autor(es)
- Kembro, Jackelyn Melissa; Cortassa, Sonia; Lloyd, David; Sollot, Steven; Aon, Miguel
- Afiliación(es) del/de los autor(es)
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Kembro, Jackelyn Melissa. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Kembro, Jackelyn Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.
Cortassa, Sonia. National Institutes of Health. NIH · NIA Intramural Research Program; Estados Unidos.
Lloyd, David. Cardiff University. School of Biosciences 1; Inglaterra.
Sollot, Steven. Johns Hopkins University. Laboratory of Cardiovascular Science; Estados Unidos.
Aon, Miguel. Johns Hopkins University. Laboratory of Cardiovascular Science; Estados Unidos.
- Resumen
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Numerical integration of the ME-R model equations was performed with MatCont 2.4 in MATLAB 7.1, until steady-state solutions were obtained (i.e., when the magnitude of each time derivative was -10). Time series with duration of at least 6e6 ms were constructed by numerical integration of model equations. To allow transient states to vanish, the system was computed during a time lapse of 2 e8 ms. The solutions were then evaluated with the function deval.m in MATLAB R2017a to obtain a time series with constant sampling frequency at 1ms. All studies were performed using the parameter setting optimized in our previous work (Kembro et al. 2013. Biophys J 104(2):332-343; Kembro et al., 2014. Front Physiol 5:257), with ADPm = 0.01mM, i.e. consistent with energized mitochondria under state 4 respiration. The two .txt file represents the time reference and the time series of variables output of the model, in order from left to right column:1) Mitochondrial Ca+;2) Mitochondrial ADP; 3) Membrane potential; 4)Mitocondrial NADH; 5) Mitochondrial H+; 6) Mitochondrial Phosfate, Pi; 7) Isocitrate; 8) a-ketoglutarate; 9) Succinyl CoA; 10) Succinate; 11) Fumarate; 12) Malate; 13) Oxaloacetate; 14) NADPH; 15) Mitochondrial superoxide; 16) Extramitochondrial superoxide; 17) Mitochondrial hydrogen peroxide; 18) Extramitochondrial hydrogen peroxide; 19) Mitochondrial GSH; 20) Extramitochondrial GSH; 21)Mitochondrial GSSG; 22) Mitochondrial TrxSH2; 23) ExtramitochondrialTrxSH; 24)Mitochondrial PSSGm; 25) Extramitochondrial PSSG
- Año de publicación
- Idioma
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inglés
- Formato (Tipo MIME)
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application/zip
- Clasificación temática de acuerdo a la FORD
- Ciencia veterinaria
- Materia
- Animal Physiology - Systems; Molecular Biology; Superoxide dismutase activity; Respiration; Oxidative stress effect; Oxidative stress enzymes;
- Condiciones de uso
- Disponible en acceso abierto
- Repositorio digital
- Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba
- Identificador alternativo
- https://doi.org/10.6084/m9.figshare.1514982.v1
- Publicación referenciada
- http://hdl.handle.net/hdl/11086/17030
Citación
Kembro, Jackelyn Melissa Cortassa, Sonia Lloyd, David Sollot, Steven Aon, Miguel (): Chaotic fluctuations in mitochondrial function under oxidative stress. SOD2 concentrations of 0.0216733 mM. Universidad Nacional de Córdoba, http://hdl.handle.net/11086/16912.