Gene expression and gene silencing data of Varroa destructor

Registro completo

Título

Gene expression and gene silencing data of Varroa destructor

Autor(es)

Scannapieco, Alejandra Carla

Afiliación(es) del/de los autor(es)

Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina

Resumen

The ectoparasite Varroa destructor is the main threat to the western honeybee Apis mellifera, worldwide. Current acaricide-based controls have usually generated mite resistance, thus developing novel sustainable control strategies for V. destructor in honeybee colonies is a priority. To investigate the potential of RNA interference (RNAi) for controlling the mite, we examined the effects of silencing candidate genes which potentially play a key role in V. destructor survival and reproduction. We synthesized double-stranded RNA (dsRNA) of six candidate genes (ptch1, ap-1, larp6, chisal, vg1 and vg6) and used a novel topical method with acetone to individually deliver the dsRNA into Varroa mites. Survival and successful reproduction were assessed in silenced and control mites using a semi-field rearing method. A significant lower expression was observed for all candidate genes in mites subjected to dsRNA treatments compared to controls, with high knockdown efficiencies ranging from 89.5 to 97.4%. Significant differences were observed in fertility of mites treated with dsRNA targeting the ptch1, ap-1, and vg1 genes compared to control mites. These genes are related to oocyte maturation and intraovarian embryogenesis. The silencing of chisal gene led to a significant increase in mite mortality compared to controls. Our findings provide insights into the roles of genes and molecular pathways associated with mite reproduction, offering possible targets for the control of V. destructor through RNAi in the future.

Año de publicación

2025

Idioma

inglés

Formato (Tipo MIME)

application/vnd.openxmlformats-officedocument.spreadsheetml.sheet

Clasificación temática de acuerdo a la FORD

Ciencias biológicas

Condiciones de uso

Parcialmente restringido. Para acceder a los datos contactate con el repostiorio. Datos sujetos al derecho de propiedad intelectual

Repositorio digital

CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas

Identificador de proyecto

Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica/PICT 2020-2564

Identificador de proyecto

Instituto Nacional de Tecnología Agropecuaria/PICT 2020-2564