{"id":"CONICETDig_f892a4ce3af54ad60a040f2ee7624e26","dc:title":"El tejido adiposo peritumoral renal sufre un proceso de pardeamiento y estimula la expresi\u00f3n de marcadores de transici\u00f3n epitelio-mesenquimatosos en c\u00e9lulas renales humanas","dc:creator":"Caron, Ruben Walter","dc:date":"2024","dc:description":["Tumor cells can interact with neighboring adipose cells and adipocyte dedifferentiation appears to be an important aspect of tumorigenesis. We evaluated the size of adipocytes in human adipose explants from normal (hRAN) and kidney cancer (hRAT); changes in the expression of WAT and BAT\/ beige markers in hRAN and hRAT; the expression of epithelial-mesenchymal transition (EMT) cell markers in human kidney tumor (786-O, ACHN and Caki-1); and non-tumor (HK-2) epithelial cell lines incubated with the conditioned media (CMs) of hRAN and hRAT. We observed that hRAT adipocytes showed a significantly minor size compared to hRAN adipocytes. Also, we observed that both Prdm16 and Tbx1 mRNA and the expression of UCP1, TBX1, PPAR\u03b3, PCG1\u03b1, c\/EBP\u03b1 LAP and c\/EBP\u03b1 LIP was significantly higher in hRAT than hRAN. Finally, we found an increase in vimentin and N-cadherin expression in HK-2 cells incubated for 24 h with hRAT-CMs compared to hRAN- and control-CMs. Furthermore, desmin and N-cadherin expression also increased significantly in 786-O when these cells were incubated with hRAT-CMs compared to the value observed with hRAN- and control-CMs. We observed a significant decrease in E-cadherin expression in the ACHN cell line incubated with hRATCMs versus hRAN- and control-CMs. However, we did not observe changes in E-cadherin expression in HK-2, 786-O or Caki-1. The results obtained, together with the results previously published by our group, allow us to conclude that perirenal white adipose tissue browning contributes to tumor development in kidney cancer. In addition, hRAT-CMs increases the expression of mesenchymal markers in renal epithelial cells, which could indicate a regulation of EMT due to this adipose tissue."],"dc:format":["application\/octet-stream"],"dc:language":["eng"],"dc:type":"dataset","dc:rights":["info:eu-repo\/semantics\/restrictedAccess","Protecci\u00f3n de datos personales (Ley 25.326)"],"dc:relation":["info:eu-repo\/grantAgreement\/Ministerio de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n Productiva. Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica. Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica\/","info:eu-repo\/grantAgreement\/\/"],"dc:identifier":"https:\/\/repositoriosdigitales.mincyt.gob.ar\/vufind\/Record\/CONICETDig_f892a4ce3af54ad60a040f2ee7624e26"}