Data Statement molecular identification of Lactiplantibacillus plantarum with antimicrobial activity against shiga toxin-producing Escherichia coli

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Título

Data Statement molecular identification of Lactiplantibacillus plantarum with antimicrobial activity against shiga toxin-producing Escherichia coli

Autor(es)

Etcheverría, Analía Inés; Ruiz, María Julia

Afiliación(es) del/de los autor(es)

Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Ruiz, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina

Resumen

Aims: The aim of the present work was to characterize Lactiplantibacillus sp. LP5 strain, isolated from pork production, and identify bacteriocin-like inhibitory substances produced by this strain. Methods and Results: In this study, LP5 was identified by specie-specific PCR and 16S rDNA sequencing. Additionally, bacterial growth kinetics, antimicrobial activity, detection of the genes related to plantaricins production and genetic expression of plantaricins, were determined. Lactiplantibacillus sp. LP5 was identified as Lactiplantibacillus plantarum. The well-diffusion test using cell-free supernatants (CFS), neutralized CFS, CFS treated with catalase and CFS treated with proteinase K showed that inhibitory effects on a Shiga toxin-producing E. coli (STEC) strain were produced by bacteriocins. The PCR technique allowed the detection of genes encoding E/F plantaricins, as well as J/K and whole genome sequencing and bacteriocin mining analysis allowed us to confirm the presence of these plantaricins. Conclusions: We can conclude that the inhibitory effect of L. plantarum LP5 isolated from pigs against STEC EDL933 strain could be associated with the bacteriocins production and represents a potential use as a probiotic strain. Significance and Impact of Study: In the food industry, there is a growing interest for the use of natural antimicrobial agents to kill or inhibit growth of frequent and important pathogens. Bacteriocins are one of the possible candidates. Currently, nisin and pediocin are the only approved bacteriocins for food industrial use. Therefore, this study will provide valuable information on a plantaricin-producing bacterium with safe pathogen inhibitory potential for its application in the food industry.

Año de publicación

2023

Idioma

inglés

Formato (Tipo MIME)

application/vnd.openxmlformats-officedocument.wordprocessingml.document

Clasificación temática de acuerdo a la FORD

Ciencia veterinaria

Condiciones de uso

Acceso restringido por un tiempo determinado. Estará disponible en: bajo licencia Creative Commons https://creativecommons.org/licenses/by-nc-sa/2.5/ar/

Repositorio digital

CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas

Identificador de proyecto

Consejo Nacional de Investigaciones Científicas y Técnicas/PICT 2015-2666

Identificador de proyecto

/PICT 2015-2666