Efecto del megacariocito sobre el estroma medular

Registro completo

Título

Efecto del megacariocito sobre el estroma medular

Autor(es)

Lev, Paola Roxana

Afiliación(es) del/de los autor(es)

Lev, Paola Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina

Resumen

Bone marrow stromal cells provide a proper environment for the development of hematologic lineages. The incorporation of different stromal cells to in vitro culture systems would be an attractive model to study megakaryopoiesis and thrombopoiesis. Our objective was to evaluate the participation of different types of stromal cells on in vitro megakaryopoiesis, thrombopoiesis and megakaryocyte (MK) survival. CD34-positive progenitors from umbilical cord blood were differentiated into MK precursors and then co-cultured with umbilical vein endothelial cells (HUVEC), bone marrow mesenchymal stem cells (MSC), skin fibroblasts (SF) (all human) or mouse fibroblast cell line (L929). The number of MKs (CD61-positive cells) was increased in the presence HUVEC and SF while L929 cells decreased total and mature MKs count. Concerning thrombopoiesis, HUVEC increased proplatelet (PP) producing MKs, while MSC, L929 and SF had the opposite effect (immunofluorescence staining and microscopic analysis). MK survival was enhanced in MSC and SF co-cultures, as assessed by pyknotic nuclei evaluation. However, HUVEC and L929 did not prevent apoptosis of MKs. Overall, our results indicate that each cell type performs distinct functions, which differentially modulate MK growth and platelet production, highlighting the relevance of considering the influence of stromal cells in MK research.

Año de publicación

2022

Idioma

inglés

Formato (Tipo MIME)

application/octet-stream
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet

Clasificación temática de acuerdo a la FORD

Medicina básica

Condiciones de uso

Disponible en acceso abierto bajo licencia Creative Commons https://creativecommons.org/licenses/by-nc-sa/2.5/ar/

Repositorio digital

CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas

Identificador de proyecto

Consejo Nacional de Investigaciones Científicas y Técnicas/11220170100034