Thyroid hormones and neurogenesis: modulation of quiescence and activation in hippocampal Neural Stem Cells.

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Título

Thyroid hormones and neurogenesis: modulation of quiescence and activation in hippocampal Neural Stem Cells.

Autor(es)

Troncoso, Mariana Elizabeth

Afiliación(es) del/de los autor(es)

Troncoso, Mariana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina

Resumen

Introduction: the adult hippocampal neurogenic niche relies on the activation of reversibly quiescent neural stem cells (NSCs). Thyroid hormones are critical regulators of neurogenesis, but their role in balancing NSC quiescence and activation has been little explored. Objective: this study examined whether thyroxine (L-T4) modulates this balance. Methodology: NSCs were isolated from 30-day-old C57BL/6J mice, cultured as neurospheres, and treated for 72 h with 1 nM or 5 nM L-T4 under two conditions: proliferation (FGF2, 20 ng/mL) or quiescence (FGF2 + BMP4, 30 ng/mL). The percentage of positive NSCs expressing Nestin, Ki67, and cyclin D1 was analysed. In proliferation conditions, both T4 concentrations increased Nestin+ cells without altering Cyclin D1, while 5 nM T4 reduced Ki67+ cells. In quiescence conditions, both doses decreased Nestin+ and Ki67+ cells, and 5 nM T4 increased Cyclin D1+ NSCs. Conclusion: These findings indicate that NSCs are sensitive to T4 levels in both proliferative and quiescent states. T4 promotes NSCs identity under proliferation conditions, but high T4 reduces proliferation, potentially preserving the NSCs pool. Under quiescence conditions, T4 may either prolong the cell cycle and reinforce quiescence or induce loss of stemness and differentiation. Altogether, our data suggest that thyroid hormones may be key modulators of the quiescence–activation balance, essential for sustaining hippocampal neurogenesis.

Año de publicación

2025

Idioma

español

Formato (Tipo MIME)

application/vnd.openxmlformats-officedocument.spreadsheetml.sheet

Clasificación temática de acuerdo a la FORD

Ciencias biológicas

Condiciones de uso

Parcialmente restringido. Para acceder a los datos contactate con el repostiorio. Datos sujetos al derecho de propiedad intelectual

Repositorio digital

CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas