{"id":"CONICETDig_76b0bb9d5a96aabde1b5bc4461c8af31","dc:title":"An\u00e1lisis molecular de Trypanosoma cruzi en sangre de piches (Zaedyus pichiy)","dc:creator":"Superina, Mariella","dc:date":"2024","dc:description":["Armadillos are considered important reservoir hosts for Trypanosoma cruzi, the causative agent of Chagas disease. The first report of T. cruzi infection in pichis (Zaedyus pichiy), a small armadillo species endemic to central Argentina and Chile, dates back to 1935. However, more recent reports on T. cruzi in this species are scarce. The objective of this study was to assess T. cruzi infection and parasite load in Z. pichiy from Mendoza Province, an area endemic to human Chagas disease. Blood samples were obtained in 2014\u20132016 from pichis from Lavalle (low Monte), Malarg\u00fce (Patagonian steppe), and San Carlos (ecotone) departments, Mendoza Province, Argentina. The detection and quantification of T. cruzi was performed through qPCR amplification using satellite primers. Of the 265 analyzed samples, 201 (76%) were positive for T. cruzi. Parasite loads varied between < 0.1\u201355.8 parasite-equivalents\/mL (par-eq\/mL), with a median of 1.1 par-eq\/mL in quantifiable samples. The prevalence was similar in Malarg\u00fce and Lavalle (85\u201394%), but significantly lower in pichis from San Carlos (50%). Animals from Lavalle captured after hibernation had significantly higher parasite loads (median 2.0 par-eq\/mL). In Malarg\u00fce, T. cruzi infection and parasite loads were significantly lower before than after hibernation in 2016. The high prevalence and low median parasite load suggest a chronic and persistent infection of T. cruzi in pichis. Regional differences and a marked increase in precipitation during 2015\u20132016 could have influenced annual and seasonal infection rates of this vector-borne disease."],"dc:format":["application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet"],"dc:language":["spa"],"dc:type":"dataset","dc:rights":["info:eu-repo\/semantics\/restrictedAccess","Datos sujetos al derecho de propiedad intelectual"],"dc:relation":["info:eu-repo\/grantAgreement\/Ministerio de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n Productiva. Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica. Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica\/PICT2014-0496"],"dc:identifier":"https:\/\/repositoriosdigitales.mincyt.gob.ar\/vufind\/Record\/CONICETDig_76b0bb9d5a96aabde1b5bc4461c8af31"}