{"id":"CONICETDig_49219e2a6c0b2d65831fea8629d06942","dc:title":"Datos de genotoxicidad, da\u00f1o celular y hematol\u00f3gicos en peces expuestos a Azoxystrobina","dc:creator":"Menone, Mirta Lujan","dc:date":"2024","dc:description":["Azoxystrobin (AZX) is a broad-spectrum systemic fungicide massively used worldwide. Its mode of action consists in the inhibition of mitochondrial respiration decreasing the synthesis of ATP and leading to oxidative stress in the target fungus. However, whether this effect occurs in non target organisms has been scarcely studied. The objectives of this work were (1) to evaluate biomarkers of oxidative stress, hematological, physiological and of genotoxicity in the native cichlid fish Australoheros facetus exposed to environmentally relevant concentrations of AZX and (2) to compare these biomarkers in different developmental stages using juvenile and adult fish (n = 6) exposed during 48 h. The exposure concentrations were 0 (negative control, C ( )), 0.05, 0.5, 5 and 50 \u03bcg\/L AZX of the commercial formulation AMISTAR\u00ae. Blood was drawn to evaluate hematology, and DNA damage through the comet assay (CA) and micronucleus test (MN). Genotoxicity was observed by mean of both biomarkers in juvenile and adult fish at 50 \u03bcg\/L AZX. Samples of liver and gills were used to determine antioxidant enzymes activity, hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents. In juvenile fish inhibition of superoxide dismutase (SOD) was observed in liver at 0.05, 5 and 50 \u03bcg\/L AZX and in gills at 5 and 50 \u03bcg\/L AZX. Glutathione- S- transferases (GST) activity increased in gills at all AZX concentrations tested. In adult fish, increase of hepatic catalase (CAT) activity at 0.5 and 50 \u03bcg\/L AZX and MDA content at 50 \u03bcg\/L AZX were observed. In gills only H2O2 content showed changes at 50 \u03bcg\/L AZX. The sensitivity showed by gills constitutes the first report about AZX toxicity in this organ. All these negative effects were observed in the range of realistic AZX concentrations, which warns of the possible consequences that it may have on the health of aquatic biota. Differences between juvenile and adult fish demonstrate the relevance of considering the developmental stage on the evaluation of biomarkers."],"dc:format":["application\/vnd.openxmlformats-officedocument.spreadsheetml.sheet"],"dc:language":["eng"],"dc:type":"dataset","dc:rights":["info:eu-repo\/semantics\/restrictedAccess","Protecci\u00f3n de datos personales (Ley 25.326)"],"dc:relation":["info:eu-repo\/grantAgreement\/Ministerio de Ciencia. Tecnolog\u00eda e Innovaci\u00f3n Productiva. Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica\/pict 2017 0980","info:eu-repo\/grantAgreement\/Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales\/pict 2017 0980","info:eu-repo\/grantAgreement\/\/pict 2017 0980"],"dc:identifier":"https:\/\/repositoriosdigitales.mincyt.gob.ar\/vufind\/Record\/CONICETDig_49219e2a6c0b2d65831fea8629d06942"}