Rickettsia in fleas of rodents from Arana (ARA), La Balandra (BLB) and Estación Experimental Julio Hirschhörn (EEJH) between 2017–2019

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Título

Rickettsia in fleas of rodents from Arana (ARA), La Balandra (BLB) and Estación Experimental Julio Hirschhörn (EEJH) between 2017–2019

Autor(es)

Espinoza Carniglia, Mario Virgilio; Lareschi, Marcela

Afiliación(es) del/de los autor(es)

Espinoza Carniglia, Mario Virgilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Lareschi, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina

Resumen

This study analyzed the presence of Rickettsia (Rickettsiales: Rickettsiaceae) in fleas (Siphonaptera) associated with cricetid rodents in periurban localities of Gran La Plata, Argentina. Rodents were captured in three localities and fleas were collected directly from their fur. After DNA extraction, fleas were prepared for microscopic identification. PCR amplification of gltA and ompB genes were performed on each individual flea to detect and identify Rickettsia. OmpB gene was sequenced and compared using nBLAST to initially identify their similarity with other sequences from Genbank. A phylogenetic tree was constructed to evaluate the relationships of the sequences obtained with others deposited in GenBank. Out of the 253 cricetids (seven species) captured, 87 fleas (four species) were collected. Of them, ten fleas (11.5%) tested positive for Rickettsia. According to nBLAST and the phylogenetic tree results, sequences of this study were identified as R. felis (Bouyer et al. 2001). Those sequences were obtained only from five of the seven cricetid species and from two of the three localities, as follows: from Street 143 and Diagonal 630, Arana Neighborhood, La Plata District: Polygenis (Polygenis) axius axius (Jordan & Rothschild, 1926) (n=3) and Polygenis (Neopolygenis) atopus (Jordan & Rhotschild, 1922) (n=1); and from Balneario La Balandra, Berisso District: Polygenis (Polygenis) axius axius (n=4), Polygenis (Neopolygenis) atopus (n=1), and Craneopsylla minerva minerva (Rothschild, 1903) (n=1). Our results confirm the presence of R. felis in fleas parasitizing cricetids in the periurban area of Gran La Plata, which could represent a risk in public health.

Año de publicación

2025

Idioma

inglés

Formato (Tipo MIME)

application/vnd.openxmlformats-officedocument.spreadsheetml.sheet

Clasificación temática de acuerdo a la FORD

Otras ciencias naturales

Condiciones de uso

Disponible en acceso abierto bajo licencia Creative Commons https://creativecommons.org/licenses/by-nc-sa/2.5/ar/

Repositorio digital

CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas

Identificador de proyecto

Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica/PICT2015-1564

Identificador de proyecto

Universidad Nacional de La Plata/PICT2015-1564

Identificador de proyecto

Consejo Nacional de Investigaciones Científicas y Técnicas/PICT2015-1564